Transdermal therapeutic System for the Administration of Peptides

ABSTRACT

The invention relates to a transdermal therapeutic system (TTS), which is suited for the administration of a peptide to a patient through skin treated with ablation. The transdermal therapeutic system includes a back layer and an active substance-containing layer that contains at least one peptide and a carrier substance, preferably as a textile web material.

The subject matter of the present invention is a transdermal therapeuticsystem (TTS) for administering peptides and other molecules of highmolecular weight. Particularly suitable in this respect are thosepeptides which can be used as active pharmaceutical ingredients. Theseinclude, in particular, the peptide hormones, especially FSH.

Transdermal therapeutic systems (TTS) as pharmaceutical administrationforms have been known for a long time. For the transdermaladministration of active pharmaceutical ingredients by means of TTS, thestratum corneum (SC), the outermost layer of the skin, in the majorityof cases constitutes the real barrier for the permeability and for therate of passage of the active pharmaceutical ingredient.

Peptides and proteins and also other high-molecular molecules, with amolecular weight of more than 500 daltons—such as, for example,tacrolimus, heparin, and numerous salts of betamethasone—are generallynot absorbed transdermally, owing to their molecular size and to theirphysicochemical properties.

Moreover, the majority of peptides possess a low oral bioavailabilityand are subject to severe, proteolytic degradation in thegastrointestinal tract. For these reasons, peptides are commonlyadministered parenterally, bypassing the gastrointestinal tract. Thisinvolves injections or infusions which are administered below the skin,into the muscle or directly into the bloodstream.

The transdermal route here would offer a noninvasive alternative—withhigh patient compliance—to this invasive, parenteral administration.Consequently there are numerous approaches to facilitating thepermeability of the skin for molecules having a molecular weight of morethan 500 daltons. These approaches include, primarily, the use ofpermeation enhancers or the additional use of heat.

Another technique for making molecules with poor skin transit amenableto transdermal administration is to facilitate the passage of an activeingredient of this kind through the stratum corneum by partly destroyingor removing this layer beforehand. These techniques, referred to as“skin ablation”, use thermal or mechanical energy in order to effectpartial destruction or removal of the stratum corneum and hence tocreate direct channels into the living epidermis. The permeability ofthe skin is increased and the transdermal absorption ofhigh-molecular-weight molecules can therefore be made possible.

As a result of this pretreatment of the skin, moreover, it is alsopossible for hydrophilic active ingredients to be administeredtransdermally, the transdermal route having hitherto been closed to suchingredients on account of their hydrophilicity. Ingredients contemplatedhere include, for example, fentanyl citrate, granisetrone HCl, Nadiclofenac, and apomorphine sulfate. Furthermore, the TTS area ofexisting TTS systems can be reduced significantly, for the same bloodlevels, by means of skin ablation pretreatment.

The skin ablation technique commonly generates a multiplicity ofmicrochannels through the stratum corneum, and yet the percentage“perforated” proportion of the treated skin area is relatively small. Adescription of the laser skin ablation technique is present in WO2007/039646.

Follicle-stimulating hormone (FSH, follitropin) is a gonadotropichormone of the anterior lobe of the hypophysis, and is also calledfollicle maturation hormone, gonadotropin A, prolan A or thylakentrin.Human FSH is an acidic glycoprotein (isoelectric point 4.5) with a 16%carbohydrate fraction and a molecular weight of around 34 000 daltons.Its a-polypeptide chain (with 92 amino acid residues) is virtuallyidentical with that of chorio(nic)gonadotrop(h)in. The 3-chain, which isspecific for FSH, contains 111 amino acid residues. FSH promotes growthand development of the gonads and incites them to hormone synthesis. Inwomen, it plays a part in the menstrual cycle, by causing a new follicleto mature and to produce estradiol. In the human gonads, it stimulatesthe formation of spermatogenic cells. For the use described here, it ispossible to use FSH from natural sources or recombinant FSH.

FSH has a relatively short half-life. In the context of thesuperovulation of ruminants, hypophyseal extracts have been much used,and then, however, may contain not only FSH but also varying amounts ofLH (luteinizing hormone). Today, recombinant FSH products are alsoavailable commercially (Gonal Puregon®).

Somatropin (also: somatotropic hormone, STH, GH) is a species-specifichormone which is formed in the anterior lobe of the hypophysis and isresponsible for the growth process. The human growth hormone, alsocalled HGH (hypophyseal or human growth hormone), is an individualpolypeptide, with a molar mass of about 21 500, composed of 191 aminoacids with 2 disulfide bridges. In terms of its composition, humansomatotropin is closely related to placental lactogen and also toprolactin. In liver and kidney, somatotropin causes the excretion ofinsulinlike growth factors which are responsible for much of the effectsof somatropin. Somatotropin secretion is inhibited by somatostatin, andstimulated by the releasing hormone somatoliberin (SRF or SRH or GH-RFor GH-RH) from the hypothalamus.

Dwarfism caused in children by the absence or under-production ofsomatotropin can be regulated by supply of human growth hormone, whichhas since been produced recombinantly in the USA as Protropin®(Genentech) and—with a different amino acid residue—Humatrope® (EliLilly). Other medical applications for somatotropin might arise in thecase of burns, signs of aging, osteoporosis, cardiovascular disorders,and obesity.

The known products, however, possess certain disadvantages, which areattributable in particular to the low stability of the peptides insolution, this low stability being common knowledge.

Molecules of high molecular weight have to date been closed off fromtransdermal administration as a result of their physicochemicalproperties. Transdermal administration of these molecules is madepossible only by pretreatment of the skin.

Lastly, injection itself may be accompanied by difficulties, which lieprimarily in pain during application, a risk of injury, and the risk ofinfections.

It is an object of the present invention to provide a transdermaltherapeutic system (TTS) for the administration of peptides and othermolecules with poor skin access.

In order for the TTS to be stable on storage at room temperature and tohave little susceptibility to microbes, it ought to include as littlewater as possible.

The TTS here is to be applied to an area of skin of which beforehand atleast a subregion of the stratum corneum has been destroyed or removed.

The intention in particular is to manufacture a TTS with the activeingredient follitropin (FSH; follicle-stimulating hormone) and/or one ofits pharmaceutically acceptable salts, with which this peptide can beadministered through the skin in therapeutic doses to a patient.

The skin is preferably to be skin which has undergone an “ablative”pretreatment, where a proportion of the stratum corneum has beenremoved.

The intention here is not only to avoid the route of administration byinjection. The TTS itself is as far as possible to be equipped withoutmicroinjection needles, microblades and/or other needles and barbs, inorder to avoid or rule out additional mechanical injury to the stratumcorneum. However, where appropriate, the TTS may also be furnished withconstruction elements of these kinds.

It is also the intention that the peptide can be applied by means of thetransdermal therapeutic system as part of a long-term application.

The product is also to be amenable to production in a simple andcost-effective way.

The object is achieved by means of a transdermal therapeutic system(TTS) for administering peptides, which comprises an active ingredientlayer which comprises a peptide and a preferably hydrophilic carriersubstance for the peptide.

The TTS may further comprise a backing layer which is impermeable to thepeptide. In one preferred embodiment the backing layer is coated on theside facing the active ingredient layer with a water-insoluble,pressure-sensitively adhesive polymer. A backing layer of this kindpreferably possesses an area which is greater than the area of theactive ingredient layer. In such a TTS, the backing layer forms an“overpatch” which ensures the reliable adhesion of the TTS on the skin.

The active ingredient layer may comprise further excipients whichstabilize the active ingredient, preferably buffer substances or sugars,but also stabilizers and preservatives.

The TTS may also comprise at least one further, additional layer ofpressure-sensitive adhesive, which is substantially free from activeingredient and is pressure-sensitively adhesive. An additional layer ofpressure-sensitive adhesive of this kind ensures reliable adhesion ofthe TTS on the skin in the event that the active ingredient layer isnot, or not sufficiently, pressure-sensitively adhesive.

The TTS may comprise active peptidic ingredients, more particularlypeptides having a molecular weight of greater than 1 500 Da.

In one particular embodiment, the TTS comprises the active ingredientfollitropin and/or at least one of its pharmaceutically acceptablesalts.

In another particular embodiment, the TTS comprises the activeingredient somatotropin.

A “transdermal therapeutic system” (TTS) is a product of laminarconstruction. In its simplest embodiment it consists of a backing layer,an active ingredient layer, and a protective sheet which lines theactive ingredient layer before the TTS is employed. In this kind ofsimple construction, the active ingredient layer is preferably madepressure-sensitively adhesive. If, however, the bond strength of theactive ingredient layer is not sufficient, the TTS may feature anadditional layer of pressure-sensitive adhesive.

This additional layer of pressure-sensitive adhesive may be disposedbetween the active ingredient layer and the protective sheet.

In one preferred embodiment, the additional layer of pressure-sensitiveadhesive is sited between the active ingredient layer and the backinglayer. In this case, in at least one section along the sidemargin/margins of the active ingredient layer, the layer ofpressure-sensitive adhesive protrudes beyond the active ingredientlayer. The additional layer of pressure-sensitive adhesive then acts asan “overpatch” during the application of the TTS, ensuring reliableadhesion to the skin. The TTS may also possess a membrane which controlsthe rate of emergence of the active ingredient from the activeingredient layer. The membrane is therefore sited on the side of theactive ingredient layer that is facing the skin during the applicationof the TTS.

The TTS itself may, finally, possess a needle layer, which comesdirectly into contact with the skin and is furnished on its bottom facewith microinjection needles (i.e., hollow needles for the flow passageof active ingredient), microblades (for scoring the uppermost layers ofskin), needles (for perforating the uppermost layers of skin) and/orbarbs (for anchoring in the skin). In one preferred embodiment, however,the TTS is furnished without such a layer.

In another embodiment, the transdermal therapeutic system may comprisemore than one active ingredient layer. These active ingredient layersmay be disposed one above another (forming an at least two-layerlaminate) or next to one another. In the case of a TTS of this kindhaving more than one active ingredient layer, the individual layers mayhave the same construction or different constructions. In “multilayersystems” of these kinds, however, these layers differ preferably on thebasis of their composition or of the active ingredient used.

The active ingredient layer may also be present in the form of aliquid-filled pouch or liquid-filled chamber, in which the activeingredient is present in dissolved, dispersed or suspended form.

Finally, the active ingredient in the active ingredient layer may bepresent in liquid microresevoirs, which are in dispersion in the activeingredient layer. With the TTS described here it is possible withpreference to administer active peptide ingredients by the transdermalroute. The technical teaching, however, can in principle also beutilized for other physiologically active substances, including moreparticularly those which have been hitherto unavailable for transdermaltherapy (hydrophilic active ingredients) or possess a molecular mass ofmore than 500, preferably more than 1500 daltons.

“Peptides”, for the purposes of the present description are amino acidcondensation products that are linked in acid amide fashion by peptidebonds. Where the molecules are constructed from two amino acid residues,they are also referred to as dipeptides; in the case of three or more,as tripeptides, tetra-, pentapeptides etc. Peptides having 2-10 aminoacid residues are therefore generally referred to collectively asoligopeptides, those with 10-100 as polypeptides. The transition fromthe latter to the higher-molecular-weight proteins is, however, notprecisely defined. Peptides having bonds between the pendant aminogroups of diaminocarboxylic acids and pendant carboxyl groups ofaminodicarboxylic acids instead of the customary peptide bonds betweenthe α-amino group and the carboxyl group are called isopeptides; theadditional bonds originating from polyfunctional amino acids such asglutamic acid, aspartic acid, lysine, and arginine are responsible forthe formation of peptide network structures.

The preferred peptides include peptide hormones. These are peptides ofhigh physiological activity which develop hormone or hormonelikeeffects. Generally speaking, the peptide hormones are oligopeptides andpolypeptides (having up to 100 amino acids), but occasionally are alsohigher-molecular-weight proteins (proteohormones). These include theglandular peptide hormones of the hypophysis (e.g.: corticotrophin,follitropin, lutropin, melanotropin, prolactin, somatotropin,thyrotropin, oxytocin, vasopressin), the releasing hormones andinhibiting factors of the hypothalamus, the peptide hormones frompancreas, stomach or gut (e.g.: glucagon, insulin, somatostatin,secretin, gastrin, cholecystokinin), from the thyroid gland (e.g.^(H)calcitonin, parathyrin). Certain oligopeptides have not only aconventional hormone activity but also growth factor activity,neurotransmitter activity or neuromodulator activivity (mediators).Examples of such include the endogenous opiates, enkephalins andendorphins.

The peptides can be used preferably in the form of a pharmaceuticallyacceptable salt.

Classed among the peptides in the sense of this description are not onlynatural peptides and peptide hormones but also nature-identical and/ormodified (that is, produced synthetically) peptides and peptidehormones, conjugated proteins (i.e., glycopeptides and glycoproteins,lipoproteins, metalloproteins, and others.

“Skin” means the normal, intact skin of a human being or mammal. Theskin has a layered construction and consists—as seen from outside toinside—of epidermis, dermis, and subcutis. Within these threecomponents, the skilled person may distinguish further layers.

In the case of the epidermis, five layers are distinguished: the hornylayer (stratum corneum), shiny layer (stratum lucidum), granular layer(stratum granulosum), spiny cell layer (stratum spinosum), and basallayer (stratum basale).

“Ablatively treated skin” means the normal, intact skin of a human beingof whose epidermis the stratum corneum has—at least partly—beendestroyed or removed. In this area of ablatively treated skin, the“proportional area of normal, intact skin of whose epidermis the atleast the stratum corneum has been destroyed or removed” (correspondingto the sum of the areas X in FIG. 2) relative to the “total normal,intact skin on whose epidermis the stratum corneum remains”(corresponding to the area A in FIG. 2) may be below 50%, preferablybelow 20%, and more preferably below 10%. The sections of the epidermisat which the stratum corneum has been removed may be irregular in shape.Preferably, however, they are of defined shape and area. Suitable shapescontemplated include rectangles, hexagons, octagons, squares, circles,and spots. The sections of the epidermis which are removed by ablativetreatment have a depth such that at least the stratum corneum is removedat the locations in question and so the “microchannels” are formedbeneath the areas X (cf. FIG. 2). The sections of the epidermis removedby ablative treatment are preferably, however, not to extend any deeperthan down to the dermis. This can be achieved by means of correspondingadaptation of the laser power and simultaneous check measurements.

The term “transdermal” refers to the route of administration through theskin of a human being or mammal. Skin here means both the normal, intactskin and also the “ablatively treated skin” in the sense of the abovedefinition.

Substances contemplated as the “carrier substance” for the activeingredient layer include substances which behave compatibly in relationto the at least one peptide. It is known that, with peptides, not onlychemical influences, such as, for example, acids, salts or organicsolvents, but also physical exposures, such as high or low temperaturesor else pressure, may alter the secondary and tertiary structure andhence ultimately, also the quaternary structure (denaturing). Denaturingmay also cause changes in the physical and physiological properties ofthe peptides. In the case of chemical cleavage of the peptides(proteolysis), fragments are produced from them, and are calledpeptones.

As far as the requirements concerning the compatibility of the carriersubstance are concerned, this means that, when the peptide is imbeddedinto the carrier substance, there must be no interaction with thepeptide that lead to any such change in the structure of the peptide orto any deterioration otherwise originating of its pharmacologicalproperties.

The effect of the carrier substance is that the at least one peptide isdistributed uniformly in the active ingredient layer. The carriersubstance preferably has the effect that the peptide molecules arepresent individually, i.e., in the form of a true “solution”.

It has emerged that suitable carrier substances are more particularlythose which are “hydrophilic”. By hydrophilic (“water-loving”) is meantthe capacity to bind water or to penetrate water and, in a furthersense, “to be wetted effectively by water”.

The carrier substance may be present in the active ingredient layer inthe form of fibers, powder or a film. The carrier substance preferablyforms a film having a constant layer thickness. This layer thickness maybe between 20 and 200 μm, preferably between 30 and 80 μm.

In another preferred embodiment, the carrier substance takes the form ofa sheetlike textile structure, preferably as a nonwoven composed ofindividual fibers, or else in the form of a woven or knitted fabric ofyarn. In these cases the carrier substance is not water-soluble.

The active ingredient layer may comprise “buffers” in order to maintaina defined pH therein and to increase the stability of the activeingredient. Buffer systems and the pH values which can be set using themare known to the skilled person. For FSH, a buffer which ensures a pH ofapproximately 7 is preferred.

Layers contemplated as the “backing layer” are occlusive andnonocclusive layers, with the occlusive layers being preferred. Theselayers are constructed of films/foils, woven and/or knitted fabrics,with films/foils being preferred. The materials involved are natural orsynthetic polymers and metals. Particularly preferred are compositematerials comprising synthetic polymers and metals in the form oflaminates. The backing layer is preferably flexible and impervious forthe active ingredient.

The “active ingredient layer” comprises as already stated—at least onepeptide and at least one carrier substance for the peptide. It may havean area of 1 to 100 cm², preferably of 2 to 80 cm², and more preferablybetween 4 to 20 cm². The thickness of the active ingredient layer may bebetween 10 and 200 μm, preferably between 15 and 90 μm, more preferablybetween 20 and 80 μm.

The “concentration” of the at least one peptide in the active ingredientlayer is heavily dependent on the therapeutic indication, on theactivity of the peptide in question, and on its molecular weight. Theconcentration may therefore vary within wide ranges and in the activeingredient layer may be between 0.1 to 99% by weight, preferably between30 and 70% by weight.

In order for the active ingredient layer which comprises a peptide and acarrier substance for the peptide to be furnished “pressure-sensitivelyadhesively”, it may be admixed with at least one “pressure-sensitiveadhesive”. The pressure-sensitive adhesives that are suitable are setout later on below. Another possibility involves furnishing the activeingredient layer pressure-sensitively adhesively by addition ofplasticizers, tackifiers, etc. Especially when the carrier substance ishighly hydrophilic, it is advantageous to use hydrophilic tackifierssuch as pantothenyl alcohol, honey, low-molecular-weight carbohydrates(such as sucrose, glucose, fructose) and derivatives thereof (such assucrose acetate isobutyrate, for example), and combinations thereof.

In one particular embodiment the active ingredient layer may comprisewater. The water content (residual moisture content), however, ispreferably low, in order not to jeopardize the mechanical stability ofthe active ingredient layer and to minimize other risks—moreparticularly microbiological risks—due to the presence of water. The“water content” in the active ingredient layer is preferably below 20%,more preferably below 10%, and very preferably below 5%.

The additional “pressure-sensitively adhesive layer” may be constructedfrom the “pressure-sensitive adhesives” that are known to the skilledperson. Pressure-sensitive adhesives are able to induce “wetting”,producing sufficient forces of adhesion, at room temperature, withoutactivation by solvent or heat, solely by being pressed onto the surfaceof the article which is to be stuck.

As “pressure-sensitive adhesives” it is possible to use “polymers” whichby virtue of the composition of their monomers possesspressure-sensitively adhesive properties. These include synthetic rubberand natural rubber, butyl rubber, styrene-butadiene copolymers,ethylene-vinyl acetate copolymers, acrylonitrile copolymers,polychloroprene, polyisobutylene, polyvinyl ethers,styrene-butadiene-styrene block polymers, styrene-isoprene-styrene blockpolymers, polyacrylates, polyesters, polyurethanes, and polysiloxanes.The adhesive properties of the polymer obtained in the polymerizationcan be modified by functional groups in the monomers of these polymers.The polymers are water-insoluble.

Another way of modifying the adhesive properties of these statedpolymers is afforded by the adaptation of the adhesive formula to thedesired properties through addition of additives such as resins,plasticizers, tackifiers, fillers and/or stabilizers.

Particularly suitable polymers having pressure-sensitively adhesiveproperties are polyacrylates, polyisobutylenes, silicones.

It is preferred to use those pressure-sensitive adhesives which arenotable for their high physical compatibility with the peptides andwhich at the same time do not trigger any instances of skin irritation,allergies or sensitization in use.

As the “protective sheet” in the transdermal therapeutic system it ispossible to use the films that are known to the skilled person, such assiliconized polyester films, for example.

The use of the transdermal therapeutic system (TTS) which comprises anactive ingredient layer and at least one peptide and at least onecarrier substance for the peptide is a further solution provided by theinvention.

For this purpose, prior to the application of the TTS, the horny layer(the stratum corneum) of the skin is at least sectionally removed,preferably by means of the laser skin ablation technique. In onepreferred embodiment this ablatively treated skin has microchannels inthis area within the stratum corneum.

Subsequent application of the TTS allows transdermal absorption of thepeptide. For this purpose, the TTS is placed directly onto theablatively treated skin. The active ingredient layer, comprising thepeptide and a carrier substance for the peptide, comes to lie directlyabove the ablatively treated skin in this case.

Owing to the at least local removal of the stratum corneum, the peptideis able to reach the underlying layers of the skin and ultimately toenter the circulation transdermally. Moisture originating from thelayers of the skin below the Stratum corneum may facilitate thetransport of the peptide through the at least locally removed sectionsof the stratum corneum (i.e., through the microchannels).

The additional pressure-sensitively adhesive layer may optionally beused to effect additional fixing of the TTS on the skin.

In one particular embodiment, during the application of the “skinablation technique”, the ablatively treated skin area is marked incolor, allowing the subsequent application of the TTS to be performedwith precision and ease.

The application time of one application may be from a few hours (forexample, 2 to 6 hours) through to one or more (for example, 3 to 7)days. Repeated applications are possible as well. For this purpose, theTTS may be placed onto the ablatively treated skin on which a TTS hasalready been applied. Preferably the TTS—especially in the case of arelatively long-lasting therapeutic application—is always placed on anarea of skin treated ablatively immediately beforehand.

One particular embodiment of the invention envisages using a transdermaltherapeutic system (TTS) which comprises

-   -   a backing layer which is furnished with a pressure-sensitively        adhesive layer,    -   a layer comprising FSH, a carrier substance in the form of a        textile sheetlike structure, and    -   a protective sheet as part of a fertility therapy.

For this purpose, in a first step, first of all the skin of a femalepatient is ablatively treated by a laser skin ablation technique. Then,in a second step, a TTS with a GnRH agonist (for example, leuprolide,buserelin, nafarelin, histrelin, goserelin or deslorelin, but preferablytriptorelins) is applied to the skin location thus treated and is lefton this skin location for a relatively long time period (at least 12hours, preferably 24 to 48 hours). The GnRH agonist which is released inthis operation is delivered transdermally to the patient, and results ina lowering of the endogenous FSH level in this patient, which ispreferably measured regularly during the treatment. If the FSH level isstill above the target value (which in general is below 10 mIU/ml),these first two steps are repeated, but on a different skin location.

If the measured level is below the target value for the endogenous FSHlevel in the patient, then, in the third step, a further skin locationon the patient is treated ablatively by a laser skin ablation technique.Then in the fourth step, at this location, the TTS of the invention withthe peptide FSH is applied. The transdermal administration of FSHproduces an increase in the FSH level in the patient, and the formationof follicles, and this can be monitored preferably by ultrasoundinvestigations. In concluding steps of the method, these follicles areremoved, fertilized in vitro, and used for the patient or for a“surrogate mother”.

The method for producing a transdermal therapeutic system (TTS) foradministering peptides, comprising an active ingredient layer whichcomprises at least one peptide and at least one carrier substance forthe peptide, comprises a plurality of steps.

In the first step, the peptide is dissolved in water, preferably in acorresponding buffer. Particularly suitable solvents contemplatedinclude isotonic saline solution and aqueous buffer solutions having acorresponding pH.

Other auxiliaries may be added to this active ingredient solution, suchas, for example, stabilizers and preservatives (examples being mannitol,cyclodextrins, poloxamer (i.e., ethylene oxide-propylene oxide blockcopolymers), methionine, histidine, and mixtures thereof).

The suitable nonpolymeric auxiliaries specifically include:

-   -   polyhydric alcohols such as threitol, erythritol,        pentaerythritol, arabitol, adonitol, xylitol, sorbitol,        mannitol, dulcitol    -   monosaccharides such as arabinose, ribose, xylose, glucose,        mannose, galatose, fructose, sorbose,    -   disaccharides such as sucrose, lactose, maltose, trehalose,        cellobiose,    -   oligosaccharides such as raffinose,    -   cyclodextrins

The resulting solution is applied in the form of individual doses to thecarrier substance, which is present in the form of a sheetlike textilestructure. The resulting active ingredient layer may then be placed onthe backing layer. In one preferred embodiment, however, the carriersubstance, which is present in the form of a sheetlike textilestructure, is placed on the backing layer before the active ingredientsolution is applied, especially when this backing layer is present inthe form of an “overpatch” (i.e., when the backing layer is coated onthe side facing the active ingredient layer with a water-insoluble,pressure-sensitively adhesive polymer and possesses an area which islarger than the area of the active ingredient layer).

In a further workstep, the resulting assembly is dried, preferably attemperatures below 40° C., more preferably below 30° C., in order toremove the solvents (and also water), preferably down to a desiredresidual moisture content of 0.5 to 20%, preferably between 1 and 10%.

The individually dosed TTS are subsequently packaged.

The examples which follow serve for illustration of the invention,without restricting it.

Example 1

FSH in solution in water is mixed with an aqueous phosphate buffersolution (pH 7.0). Added to this solution are cyclodextrin, methionine,poloxamer 188, and meta-cresol for protein stabilization. This solutionis then metered with areal precision onto a sheetlike textile structure(nonwoven) which has been placed onto an overpatch.

The table below shows the composition of the resultant active ingredientlayer in the dried state:

Active ingredient layer Amount [in mg] Amount [in %] FSH (900 I.U.)0.111 0.48 cyclodextrin 20.00 86.54 meta-cresol 0.30 1.30 methionine0.10 0.43 poloxamer 188 0.10 0.43 sodium monohydrogen 1.58 6.84phosphate sodium dihydrogen 0.92 3.98 phosphate Total: 23.111 100.00

The “cyclodextrin” used in the specific case ishydroxyl-propyl-β-cyclodextrin. The material of the pressure-sensitivelyadhesive layer on the backing layer is a silicone-basedpressure-sensitive adhesive with a size of 16.5 cm², while the layercomprising active ingredient has an area of 5 cm².

Together with the sheetlike textile structure, the resulting proportionsare as follows:

Active ingredient layer Amount [in mg] Amount [in %] FSH (900 I.U.)0.146 0.46 cyclodextrin 20.00 63.2 meta-cresol 0.30 0.95 methionine 0.100.3 poloxamer 188 0.10 0.3 sodium monohydrogen 1.58 5.0 phosphate sodiumdihydrogen 0.92 2.9 phosphate sheetlike textile 8.5 26.86 structureTotal: 31.65 100.00

The active ingredient layer and the protruding edges of the layer ofpressure-sensitive adhesive are lined using a siliconized polyesterfilm.

Example 2

Specimens as per example 1 are produced, with the difference that theloading with FSH corresponds to 300 I.U./5 cm², 600 I.U./5 cm², and 1200I.U./5 cm2.

Example 3

Specimens of the transdermal therapeutic systems produced in example 2,with FSH as active ingredient, are investigated for their permeationbehaviour through laser-pretreated cow udder skin in a Franz cell. Theresults of these investigations are shown in FIG. 4.

DESCRIPTION OF THE FIGURES

FIG. 1 shows the diagrammatic structure of intact, normal skin, with anenlarged section of the outermost layer. Definitions therein are asfollows:

E=epidermis

-   -   D=dermis    -   S=subcutis    -   B=blood vessel    -   s.c.=stratum corneum    -   s.l.=stratum lucidum    -   s.gr.=stratum granulosum    -   s.sp.=stratum spinosum    -   s.b.=stratum basale

FIG. 2 shows the diagrammatic structure of ablatively treated skin.Here, A denotes the ablatively treated area of skin, and X the areas atwhich the stratum corneum has been removed.

FIG. 3 shows the diagrammatic structure of a transdermal therapeuticsystem as per example 1.

Definitions are as follows: 1=backing layer, 2=layer ofpressure-sensitive adhesive, 3=active ingredient layer, 4=protectivesheet.

FIG. 4 shows the effect of the amount of FSH on the in vitro permeationas per example 2. The permeation barrier used for the investigations wasablatively treated human skin.

1. A transdermal therapeutic system (TTS) for administering a peptideonto ablatively treated skin to a patient comprising (i) a backing layerfurnished with a pressure-sensitively adhesive layer comprising at leastone water-insoluble polymer; (ii) an active ingredient layer comprisingat least one peptide and a carrier substance in the form of a sheetliketextile structure; and (iii) a protective sheet.
 2. The TTS as claimedin claim 1, wherein the carrier substance is not water-soluble.
 3. TheTTS as claimed in claim 1, wherein the peptide is an oligopeptide, apolypeptide, a protein, an isopeptide, a peptide hormone or acombination thereof.
 4. The TTS as claimed in claims 1, wherein thepeptide is a glandular peptide hormone of a hypophysis, a releasinghormone of a hypothalamus, an inhibiting factor of a hypothalamus, apeptide hormone from a pancreas, a peptide hormone from a stomach or apeptide hormone from a gut.
 5. The TTS as claimed in claims 1, whereinthe peptide is follicle-stimulating hormone (follitropin, FSH) orsomatotropic hormone (somatotropin, STH).
 6. The TTS as claimed in claim1, wherein the peptide is in the form of a pharmaceutically acceptablesalt.
 7. The TTS as claimed in claim 1, wherein the peptide is presentin a concentration of 0.01 to 99% by weight (dry) in the activeingredient layer.
 8. The TTS as claimed in claims 1, wherein the watercontent (residual moisture content) of the active ingredient layer isbelow 20% of the total weight of said layer.
 9. The TTS as claimed inclaim 1, wherein the active ingredient layer comprises at least onesubstance from the group of nonpolymeric auxiliaries, buffers,stabilizers, and preservatives.
 10. The TTS as claimed in claim 1,wherein the active ingredient layer has an area of 1 to 100 cm² and athickness of between 10 and 200 μm.
 11. A method for producing a TTScomprising (i) a backing layer furnished with a pressure-sensitivelyadhesive layer; (ii) an active ingredient layer comprising at least onepeptide and a carrier substance in the form of a sheetlike textilestructure; and (iii) a protective sheet, said method comprising: a.placing a carrier substance having a sheetlike textile structure ontothat side of a backing layer that is coated with a water-insoluble,pressure-sensitively adhesive polymer, b. preparing an active ingredientsolution by mixing the peptide with water and optionally, at least oneauxiliary, c. applying the active ingredient solution to the sheetliketextile structure, d. drying the sheetlike textile structure and appliedactive ingredient solution to a predetermined water content of below20%, and e. lining an assembly comprising the backing layer and activeingredient layer with a protective sheet, and packaging it the linedassembly.
 12. The method of claim 11, wherein the drying takes place ata temperature below 40° C.
 13. A method for administering a peptidethrough ablatively treated skin to a patient comprising applying atransdermal therapeutic system (TTS) comprising a backing layer, whichis furnished with a pressure-sensitively adhesive layer, and an activeingredient layer comprising at least one peptide and a carrier substancein the form of a sheetlike textile structure to ablatively treated skinon a patient.
 14. A method for administering a peptide as claimed inclaim 13, where the peptide is follitropin or somatotropin and thepatient is a person receiving assistive fertility therapy.
 15. A methodof fertility treatment comprising application of a transdermaltherapeutic system (TTS) comprising a backing layer, a layer comprisingthe active ingredient FSH, and a protective sheet, comprising, in afirst step, ablatively treating the skin of a female patient by a laserskin ablation technique, applying, in a second step, a first TTS with aGnRH agonist to the skin location thus treated and leaving the first TTSwith the GnRH agonist at this skin location for a prolonged time period,the first TTS delivering the GnRH agonist released in the second steptransdermally to the patient; in a third step, ablatively treating afurther skin location on the patient by a laser skin ablation technique,and at this further skin location, in the fourth step, applying the TTSwith the active ingredient FSH thereby administering FSH transdermallyand bringing about the formation of follicles in the patient.
 16. TheTTS as claimed in claim 7, wherein the peptide is present in aconcentration of 0.1 to 50% by weight.
 17. The TTS as claimed in claim8, wherein the water content, as residual moisture content, of theactive ingredient layer is below 10% of the total weight of said layer.18. The TTS as claimed in claim 10, wherein the active ingredient layerhas an area of 2 to 80 cm2, and a thickness of between between 15 and 90μm.
 19. The TTS as claimed in claim 18, wherein the active ingredientlayer has a thickness of between 20 and 80 μm.
 20. A method forproducing a TTS as claimed in claim 11, wherein step (d) comprisesdrying to a water content of below 10%.